BeNa Culture Collection
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| Culture medium | BNCC352251 |
| Description | YM agar medium |
| Composition | Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 30 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium or sterile water and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, does not produce pigments, G+(blue purple), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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