BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352252 |
| Description | Brain Heart Infusion Agar |
| Composition | Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:13.5g, pH:7.4±0.2(25℃) |
| Growth conditions | 45 °C; 24-48 hours; aerobic |
| Subculture procedure | (1)Prepare 1–2 agar plate medium as described above. (2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3)Add 0.5 mL sterile water to the freeze dried ampoule, mix thoroughly to reconstitute the lyophilized pellet, then pipette 200 μL onto each plate and spread evenly. (4) Incubate plates under the specified growth conditions; the strain is ready for use once growth is observed. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with irregular edges, opaque, yellow on the front, light color, flat shape, rough surface, wrinkled surface, hard texture, dry texture, G+(blue purple), filamentous |
| Sharing mode | Public welfare sharing |
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