BeNa Culture Collection
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| Culture medium | BNCC387026 |
| Description | Methanosarcina Medium |
| Composition | K2HPO4:0.35g, KH2PO4:0.23g, NH4Cl:0.50g, MgSO4.7H2O:0.50g, CaCl2.2H2O:0.25g, NaCl:2.25g, Yeast extract:2.00g, Casitone:2.00g, CH3COONa:2.50g, Sodium resazurin(0.1% w/v):0.50mL, NaHCO3:2.00g |
| Growth conditions | 35 ℃; 5-7 days; anaerobic |
| Subculture procedure | ① Dissolve the Frozen vial in a water bath (35 ℃) and rapidly shake for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cover, aspirate all the dissolved solution, and transfer it into one liquid test tube (which needs to be placed in an anaerobic environment for 24 hours of deoxygenation in advance); ④ Then transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | G - (red), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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