Clostridium difficile|362776 |BNCC

BeNa Culture Collection

Clostridium difficile-BNCC
  • BNCC
  • Clostridium difficile-BNCC

Clostridium difficile

  • Price: $ 335
  • number:BNCC362776
  • MPs:
    The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis
Essential Information Related Products
Clostridium difficile
Culture medium BNCC352241
Description Columbia blood agar plate
Composition Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions 37 ℃; 48-72 hours; anaerobic
Subculture procedure ① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions -80 ℃
Safety level 2
morphology The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis
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