BeNa Culture Collection
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| Culture medium | BNCC372404 |
| Description | Reinforced Clostridial Medium (RCM) |
| Composition | Yeast Extract:3.0g, Beef Infusion Powder:10.0g, Glucose:5.0g, Pancreatic Digest of Casein:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, CH3COONa:3.0g, Agar:0.5g, pH:6.8±0.1(25℃) |
| Growth conditions | 37 ℃; 3-5 days; Strictly anaerobic |
| Subculture procedure | ① Prepare 1 tube of liquid culture medium with a diameter of 18mm, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; ③ Suck the bacterial solution back into the test tube liquid culture medium; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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