Pseudomonas aeruginosa|363610 |BNCC

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Faecalibacterium prausnitzii

Culture medium	BNCC372404
Description	Reinforced Clostridial Medium (RCM)
Composition	Yeast Extract:3.0g, Beef Infusion Powder:10.0g, Glucose:5.0g, Pancreatic Digest of Casein:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C₃H₇NO₂S.HCl):0.5g, CH₃COONa:3.0g, Agar:0.5g, pH:6.8±0.1(25℃)
Growth conditions	37 ℃; 3-5 days; Strictly anaerobic
Subculture procedure	① Prepare 1 tube of liquid culture medium with a diameter of 18mm, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; ③ Suck the bacterial solution back into the test tube liquid culture medium; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	-80 ℃
Safety level	1
morphology	G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing