BeNa Culture Collection
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| Culture medium | BNCC372557 |
| Description | Glycerol Asparagine Agar Base |
| Composition | Glycerol:10.0g, L-Asparagine:1.0g, K2HPO4:1.0g, FeSO4:1.0mg, MnCl2:1.0mg, ZnSO4:1.0mg, Agar:15.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 28 ℃; 3-5 days; aerobic |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 2-4mm, opaque, round, with irregular edges, white on the front, light in color, raised in the middle, wrinkled on the surface, rough on the surface, difficult to pick/cut, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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