BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352246 |
| Description | R2A Agar |
| Composition | Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K2HPO4:0.3g, MgSO4:0.024g, Sodium Pyruvate(C3H3NaO3):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃) |
| Growth conditions | 28 ℃; 48-72 hours; aerobic |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Cultivate the agar plate under the above cultivation conditions, and the bacterial strain can be used once it grows. The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white front, raised middle, bright surface, smooth surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, round in shape, with neat edges, opaque, grayish-white on the front, raised in the middle, bright and smooth surface, moist texture, easy to stir, G+ (blue-purple), and is a bacillus |
| Sharing mode | Public welfare sharing |
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