Colloidal Bacillus subtilis|193571 |BNCC

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Paenibacillus mucilaginosus

Culture medium	BNCC352253
Description	Silicate Bacteria Medium
Composition	Glucose:5.0g, Na₂HPO₄:2.0g, MgSO₄:0.5g, FeCl₃:0.005g, CaCO₃:0.1g, Glass Powder:1.0g, pH:7.0±0.1(25℃)
Growth conditions	30 ℃; 24-48 hours; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 2-4mm, circular, with uneven edges. The front side is grayish white, transparent, with a raised center. The surface is smooth and bright, with a moist and viscous texture. It is G+ (blue-purple), a bacillus. Purity: pure
Sharing mode	Public welfare sharing