BeNa Culture Collection
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| Culture medium | BNCC352233 |
| Description | Potato glucose agar |
| Composition | Potato Infusion Powder:6.0g, Glucose:20.0g, Agar:20.0g, pH:5.6±0.2(25℃) |
| Growth conditions | 24 ℃; 5-7 days; aerobic |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake quickly for dissolution; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each plate contains approximately 200 μL, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After spreading evenly, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The agar layer is obvious, with white mycelium and low growth. The back of the culture medium is light yellow |
| Sharing mode | Public welfare sharing |
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