Colletotrichum acutatum|228357 |BNCC

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Isaria tenuipes

Subculture procedure	① Prepare 1-2 PDA plates; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious colonies on PDA medium, white hyphae, sparse and flat, no spreading, and a light yellow color on the back of the medium
Sharing mode	Public welfare sharing

1.Description

1. Name：Isaria tenuipes

2. BNCC No.：228357

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, Potato, glucose agar medium (PDA): potato extract 1.0L, glucose 20.0g, agar 15.0g,pH natural. [Note] Potato extract: 200g of peeled potato is taken, cut into small pieces, add 1.0L of water to boil for 30min, filter out potato pieces, and supplement the filtrate to 1.0L.

2. Atmosphere：aerobic

3. Temperature： 25 ℃

4.Notes：

1.Normal culturing time, 1-2days for bacterial, 3days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization

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