Nocardia in guinea pig ear inflammation|369716 |BNCC

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Nocardia otitidiscaviarum

Culture medium	BNCC352255
Description	Streptomyces medium 2
Composition	Yeast Extract Powder:4.0g, Malt Extract Powder:10.0g, Glucose:4.0g, Agar:20.0g, pH:7.2±0.2(25℃)
Growth conditions	28 ℃; 48-72 hours; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony has a diameter of 1-2mm, irregular shape, serrated edges, opaque, yellow on the front and yellow on the back, raised in the middle, rough surface, hard texture, dry texture, does not produce pigments, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing