BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 4-5 days; 5% CO₂ |
| Subculture procedure | ① Thaw Frozen vials in a 37°C water bath, shaking vigorously to dissolve; ② Disinfect the outer surface of the vial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire thawed solution, and Agar plate onto 1-2 agar plates; ④ After uniform spreading, incubate under the specified conditions. (Keep plates moist during incubation; if plates dry out, open them in the biosafety cabinet and inoculate with appropriate amounts of peptone soy broth) |
| Storage conditions | -80°C |
| Safety level | 2 |
| morphology | Colony diameter: 0.5-1 mm, circular, with neat, opaque edges; grayish-white surface, centrally convex, smooth texture, moist consistency, easily liftable; G- (red); Vibrio |
| Sharing mode | Public welfare sharing |
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