Sediment-dwelling new sphingosine bacteria|372335 |BNCC

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Novosphingobium sediminicola

Culture medium	BNCC352246
Description	R2A Agar
Composition	Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K₂HPO₄:0.3g, MgSO₄:0.024g, Sodium Pyruvate(C₃H₃NaO₃):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃)
Growth conditions	30°C; 24-48h; aerobic;
Subculture procedure	① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Immediately crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. Thoroughly dissolve bacterial powder, then dispense 200μL onto each of two plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge for use.
Storage conditions	2-8°C
Safety level	1
morphology	Irregular shape, neat edges, opaque, light color, yellow upper surface, central elevation, smooth texture, viscous consistency, moist texture, glossy surface, G− (red), bacillus, purity: pure
Sharing mode	Public welfare sharing