BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 45 ° C; 3-5 days; aerobic (low temperature storage can cause strain inactivation) |
| Subculture procedure | ① Prepare one 90mm solid Agar plate, one inoculation shovel, and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full. |
| Storage conditions | 28 ℃ |
| Safety level | 1 |
| morphology | The front is gray white with obvious layers of bacterial colonies spreading and growing, while the back is black |
| Sharing mode | Public welfare sharing |
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