Intracellular mycobacteria|378483 |BNCC

Essential Information Related Products

Mycobacterium intracellulare

Culture medium	BNCC360851
Description	Middlebrook 7H10 Agar
Composition	(NH₄)₂SO₄:0.5g, KH₂PO₄:1.5g, Na₂HPO₄:1.5g, Na₃C₆H₅O₇:0.4g, MgSO₄:0.025g, CaCl₂:0.5mg, ZnSO₄:0.001g, CuSO₄:0.001g, Sodium L-Glutamate(C₅H₈NNaO₄):0.5g, C₆H₈FeNO₇:0.04g, Pyridoxine Hydrochloride(C₈H₁₁NO₃.HCl):0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions	37 ℃; 5-10 days; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water or liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per Agar plate and apply evenly; ④ Cultivate the agar plate under the above cultivation conditions, and the bacterial strain can be used once it grows. The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, yellow on the front, light in color, raised in the middle, rough on the surface, dry in texture, G+(blue purple), Bacillus subtilis
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, yellow on the front, light in color, raised in the middle, rough on the surface, dry in texture, G+(blue purple), Bacillus subtilis
Sharing mode	Public welfare sharing