BeNa Culture Collection
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| Culture medium | BNCC352252 |
| Description | Brain Heart Infusion Agar |
| Composition | Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:13.5g, pH:7.4±0.2(25℃) |
| Growth conditions | 37°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 1-2 fresh plates as above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto the Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the strain is ready for use once colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 1-2 mm; circular with neat edges; opaque; pale yellow on top, yellow underneath; centrally raised; smooth surface; moist texture; non-pigmented; Gram-positive (blue-violet); bacilli; Purity: Pure |
| Sharing mode | Public welfare sharing |
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