Desnitrosomonas|379332 |BNCC

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Pseudovibrio denitrificans

Culture medium	BNCC353703
Description	2216E agar
Composition	Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC₆H₅O₇):0.1g, KBr:0.08g, NaCl:19.45g, MgCl₂:5.98g, Na₂SO₄:3.24g, CaCl₂:1.8g, KCl:0.55g, Na₂CO₃:0.16g, Na₂NO₃:0.0016g, Na₂HPO₄:0.008g, SrCl₂:0.034g, H₃BO₃:0.022g, Na₂SiO₃:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃)
Growth conditions	28°C; 24-48h; aerobic
Subculture procedure	① Thaw Frozen vials in water bath (fungi 30°C, bacteria 37°C), dissolve by rapid shaking; ② Disinfect outer surface of vial with alcohol swab, then transfer to biosafety cabinet for handling; ③ Unscrew cap, aspirate entire thawed solution, and inoculate 1-2 agar plates (approx. 200μL per Agar plate; for anaerobic bacteria, pre-deoxygenate medium in anaerobic conditions for 24h); ④ After uniform spreading, incubate under the specified conditions.
Storage conditions	-80°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, irregularly margined, opaque; grayish-white surface with central elevation; smooth, glossy surface; moist texture; easily liftable; Gram-negative (red); vibrio; purity: pure
Sharing mode	Public welfare sharing