BeNa Culture Collection
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| Culture medium | BNCC352246 |
| Description | R2A Agar |
| Composition | Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K2HPO4:0.3g, MgSO4:0.024g, Sodium Pyruvate(C3H3NaO3):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃) |
| Growth conditions | 28°C; 48-72h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Immediately crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; opaque; white upper surface with central elevation; smooth, glossy surface; viscous, moist texture; non-pigmented; Gram-positive (blue-violet); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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