BeNa Culture Collection
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| Culture medium | BNCC359963 |
| Description | Trypticase Soy Blood Agar |
| Composition | Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Sterile Defibrinated Sheep Blood:5%, Agar:15.0g, pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 48-72h; anaerobic |
| Subculture procedure | ① Thaw Frozen vials in a water bath (bacteria at 37°C), dissolve by rapid shaking; ② Disinfect outer surface of vial with alcohol swab, then transfer to safety cabinet for handling; ③ Unscrew cap, aspirate entire thawed solution, and inoculate 1-2 agar plates (approx. 200μL per Agar plate; for anaerobic cultures, pre-deoxygenate medium in anaerobic conditions for 24h); ④ After uniform spreading, incubate under the specified conditions. |
| Storage conditions | -80°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular, with regular margins; opaque, grayish-white upper surface; central elevation; smooth texture; moist consistency; easily liftable; Gram-negative (red); bacilli |
| Sharing mode | Public welfare sharing |
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