BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 3-4 days; anaerobic |
| Subculture procedure | ① Thaw Frozen vial in 37°C water bath, shake vigorously to dissolve; ② Disinfect the outer wall of the vial with an alcohol swab, then transfer to anaerobic glove box for handling; ③ Unscrew the cap, aspirate the entire thawed suspension, and inoculate 1-2 agar plates (approx. 200μL per Agar plate; for anaerobic cultures, pre-deoxygenate the medium in anaerobic conditions for 24h); ④ After uniform spreading, incubate under the aforementioned conditions. |
| Storage conditions | -80°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular, with neat margins; opaque, white upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; Gram-negative (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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