BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 28°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder completely, then dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; opaque; golden yellow upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; Gram-negative (red); short rod-shaped; purity: pure |
| Sharing mode | Public welfare sharing |
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