BeNa Culture Collection
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| Culture medium | BNCC360812 |
| Description | Concentrated sugar Chatelier agar |
| Composition | Sucrose:200.0g, NaNO3:3.0g, MgSO4.7H2O:0.5g, KCl:0.5g, FeSO4.4H2O:0.01g, K2HPO4:1.0g, Agar:15.0g, pH:6.0-6.5(25℃) |
| Growth conditions | 25 ° C; 5-7 days; aerobic; |
| Subculture procedure | ① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Spread and grow, produce a large number of spores, produce yellow spores, irregular edges, opaque, white mycelium, turn golden yellow after spore production, golden yellow on the reverse side, flat shape, rough surface, wrinkled surface, dry texture, purity: pure |
| Sharing mode | Public welfare sharing |
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