BeNa Culture Collection
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| Culture medium | BNCC384501 |
| Description | Ruminococcus Medium Base with Cellobiose |
| Composition | Tryptone:5.0g, Yeast extract:2.0g, Glucose:3.0g, Cellobiose:2.0g, Resazurin:0.001g, Na2CO3:4.0g, C3H7NO2S.HCl:0.5g, Mineral solution 1:K2HPO4:0.24g, Mineral solution 2:KH2PO4:0.24g, (NH4)2SO4:0.8g, NaCl:0.48g, MgSO4.7H2O:0.1g, CaCl2.7H2O:0.064g, Volatile fatty acid solution:1mL |
| Growth conditions | 37 ℃; 3-7 days; anaerobic |
| Subculture procedure | ① Prepare 1 test tube of liquid culture medium (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer the bacterial solution to a new liquid culture medium using a pipette or suction tube in an anaerobic environment; ④ Incubate the liquid test tube under the above cultivation conditions until the bacterial solution becomes turbid and ready for use. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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