BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC387409 |
| Description | Brucella Agar |
| Composition | Brucella Agar(BD-BBL Brand):43.0g, Horse blood:50.0mL, Hemin solution:10.0mL, Vitamin K3 solution:10.0mL |
| Growth conditions | 37 ℃; 5-7 days; anaerobic |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), short rod-shaped, purity: pure |
| Sharing mode | Public welfare sharing |
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