Bone shaped bottle fungi|386938 |BNCC

Essential Information Related Products

Apophysomyces ossiformis

Culture medium	BNCC352256
Description	Czapek Dox Agar
Composition	NaNO₃:3.0g, K₂HPO₄:1.0g, MgSO₄:0.5g, KCl:0.5g, FeSO₄:0.01g, Sucrose:30.0g, Agar:15.0g
Growth conditions	37 ℃; 48-72h; aerobic
Subculture procedure	① Prepare one 90mm solid Agar plate, one inoculation shovel, and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment (if there is paraffin, all paraffin should be sucked out), first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full.
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi grow well on Chatelier medium, producing white hyphae on the front of the medium that spread and grow around the Agar plate, while the back of the medium is white
Sharing mode	Public welfare sharing