Clostridium perfringens|387046 |BNCC

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Clostridium perfringens

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 18-24h; anaerobic
Subculture procedure	① Prepare two plates as above (equilibrated in anaerobic conditions for 24h); ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture; break open with forceps; ③ Pipette approximately 0.5 mL sterile water (pre-equilibrated anaerobically for 24h) into the lyophilized vial. Thoroughly dissolve the bacterial powder, then inoculate 2 plates with 200 μL each, spreading evenly; ④ Incubate plates under the aforementioned conditions.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 2–4 mm; circular shape with serrated edges; opaque; grayish-white upper surface; flat morphology with wrinkled texture; moist consistency; easily liftable; Gram-positive (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing