BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 18-24h; anaerobic |
| Subculture procedure | ① Prepare two plates as above (equilibrated in anaerobic conditions for 24h); ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture; break open with forceps; ③ Pipette approximately 0.5 mL sterile water (pre-equilibrated in anaerobic conditions for 24 hours) into the lyophilized vial. Thoroughly dissolve the bacterial powder, then inoculate two plates with 200 μL each, spreading evenly; ④ Incubate plates under the aforementioned culture conditions. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 1-2 mm; circular, with neat margins; opaque, grayish-white upper surface; convex center; smooth, shiny surface; moist texture; easily lifted; G+ (blue-violet); bacilli; purity: pure |
| Sharing mode | Public welfare sharing |
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