BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder completely, then dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 1-2 mm; circular, with neat edges; opaque, pale yellow on top; centrally convex; smooth surface; glossy appearance; moist texture; Gram-negative (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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