Duncan's bacteria in mouse colon|390382 |BNCC

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Duncaniella muricolitica

Culture medium	BNCC360847
Description	Cooked Meat Medium
Composition	Peptone:30.0g, Beef Extract Powder:3.0g, Yeast Extract Powder:5.0g, Soluble Starch:2.0g, Glucose:3.0g, NaH₂PO₄:5.0g, pH:7.0-7.4(25℃)
Growth conditions	37 °C; 3-7 days; anaerobic
Subculture procedure	① Dissolve the Frozen vial in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into one test tube (the culture medium needs to be deoxygenated 24 hours in advance); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	2
morphology	G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing