Bacillus subtilis|132576 |BNCC

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Paenibacillus chibensis

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it, and subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid test tubes and plates under the specified conditions; the strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat margins; opaque; pale yellow surface; centrally raised; smooth surface; glossy appearance; moist texture; easily liftable; G+ (blue-violet); bacillus
Sharing mode	Public welfare sharing