Spindle-shaped Bacillus lysine|132602 |BNCC

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Lysinibacillus fusiformis

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it, followed by crushing it with forceps; ③ Pipette 0.5 mL liquid medium into the lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid test tubes and plates under the specified conditions; the culture is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat margins; opaque; yellow upper surface; centrally raised; glossy surface; smooth texture; moist consistency; easily liftable; Gram-positive (blue-violet); bacilli
Sharing mode	Public welfare sharing