BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 30°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush contents with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve bacterial powder, then inoculate two plates (200 μL/Agar plate) with uniform smears; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular with neat margins; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); bacilli; purity: pure |
| Sharing mode | Public welfare sharing |
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