Escherichia hystrix|391061 |BNCC

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Escherichia marmotae

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C; 18-24h; aerobic;
Subculture procedure	① Prepare 1-2 fresh plates as above; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto the Agar plate and spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with neat edges; opaque, creamy white on top; centrally convex; surface smooth, shiny, and moist; non-pigmented; Gram-negative (red); bacillus; purity: pure
Sharing mode	Public welfare sharing