BeNa Culture Collection
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| Culture medium | BNCC363892 |
| Description | Modified PYG Broth |
| Composition | Pancreatic Digest of Casein:5.0g, Peptone:5.0g, Yeast Extract:10.0g, Beef Extract:5.0g, Glucose:5.0g, K2HPO4:2.0g, Tween 80:1.0ml, CaCl2.2H2O:0.01g, MgSO4.7H2O:0.02g, K2HPO4:0.04g, KH2PO4:0.04g, NaHCO3:0.4g, NaCl:0.08g, Resazurin:1.0mg, Hemin:5mg, Vitamin K1:1mg, C3H7NO2S.HCl:0.5g, pH:7.2±0.2(25℃) |
| Growth conditions | 37°C; 3-5 days; Strict anaerobic |
| Subculture procedure | ① Thaw Frozen vials in water bath (fungi 30°C, bacteria 37°C), dissolve by rapid shaking; ② Disinfect outer surface of Frozen vials with alcohol swab, then transfer to biosafety cabinet for handling; ③ Unscrew cap, aspirate entire thawed contents, and Agar plate onto 1–2 agar plates (approx. 200μL per Agar plate; for anaerobic bacteria, pre-deoxygenate medium in anaerobic conditions for 24h); ④ Spread evenly and incubate under specified conditions. |
| Storage conditions | -80°C |
| Safety level | 1 |
| morphology | G+ (blue-purple), coccoid, Purity: Pure |
| Sharing mode | Public welfare sharing |
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