Bacillus subtilis subsp. kenya|133216 |BNCC

Essential Information Related Products

Bacillus thuringiensis subsp. kenyae

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 2–4 mm Shape: Circular Margins: Irregular Transparency: Opaque Color: Light gray Elevation: Flat Surface: Rough and dull Texture: Dry
Sharing mode	Public welfare sharing