BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 25°C; 5-7 days; aerobic; |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the powder thoroughly, then dispense approximately 200μL onto each of two plates, spreading evenly; ④ Incubate plates under the specified conditions. The culture is ready for use once the fungal growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | A small filamentous fungus that grows well on comprehensive PDA medium. The medium surface appears dark green with spreading growth, while the underside is brownish-green. |
| Sharing mode | Public welfare sharing |
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