Escherichia coli|133359 |BNCC

BeNa Culture Collection

Escherichia coli-BNCC
  • BNCC
  • Escherichia coli-BNCC

Escherichia coli

  • Price: $ 186
  • number:BNCC133359
  • MPs:
    Colony diameter: 0.5–1 mm; circular, with neat edges; opaque, pale yellow on top; raised center; smooth surface; easily lifted; G− (red); bacillus
  • Packing:Agar Slant; Bacterial suspension; Agar plate
Essential Information Related Products
Escherichia coli
Culture medium BNCC352239
Description Nutrient Agar
Composition Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions 37°C; 18-24h; aerobic
Subculture procedure ① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2 mL of the bacterial suspension onto each Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions 2-8°C
Safety level 1
morphology Colony diameter: 0.5–1 mm; circular, with neat edges; opaque, pale yellow on top; raised center; smooth surface; easily lifted; G− (red); bacillus
Sharing mode Public welfare sharing
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