Escherichia coli|391242 |BNCC

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Escherichia coli (Migula) Castellani and Chalmers

Culture medium	BNCC363070
Description	Tryptic Soy Agar (TSA)
Composition	Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.2(25℃)
Growth conditions	37°C; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 plates as described above; ② Disinfect the ampoule surface, open it in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Immediately crush it with forceps; ③ Pipette 0.5 mL sterile water into the lyophilized tube. After fully dissolving the bacterial powder, dispense 200 μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 2-3 mm; circular with neat margins; opaque, creamy white surface; centrally convex; smooth texture; viscous consistency; easily liftable; Gram-negative (red); bacillus; purity: pure
Sharing mode	Public welfare sharing