Pseudomonas aeruginosa|133867 |BNCC

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Pseudomonas aeruginosa

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C, 18-24h, aerobic
Subculture procedure	(1)Prepare 1–2 agar plate medium as described above. (2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3)Add 0.5 mL sterile water to the freeze dried ampoule, mix thoroughly to reconstitute the lyophilized pellet, then pipette 200 μL onto each plate and spread evenly. (4) Incubate plates under the specified growth conditions; the strain is ready for use once growth is observed.
Storage conditions	2-8°C
Safety level	2
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Pale yellow Elevation: Central dome Surface: Bright and smooth Texture: Moist and viscous
Sharing mode	Public welfare sharing