Streptococcus infantum|391619 |BNCC

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Streptococcus infantis

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 24-48h; 5% CO₂
Subculture procedure	① Prepare 1-2 plates as described above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder completely, then dispense approximately 200μL onto each of two plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5-1 mm; circular with neat margins; opaque; yellow surface; central elevation; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); cocci; purity: pure
Sharing mode	Public welfare sharing