BeNa Culture Collection
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| Culture medium | BNCC352233 |
| Description | Potato glucose agar |
| Composition | Potato Infusion Powder:6.0g, Glucose:20.0g, Agar:20.0g, pH:5.6±0.2(25℃) |
| Growth conditions | 28°C; 5-7 days; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under specified conditions until colonies emerge for use. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony growth is pronounced, forming a green spore layer with possible exudate production; the reverse side of the medium appears brownish. |
| Sharing mode | Public welfare sharing |
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