Staphylococcus aureus subspecies aureus|134242 |BNCC

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Staphylococcus aureus subsp. aureus

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid test tubes and plates under the specified conditions; the strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 0.5–1 mm; circular, with neat edges; opaque; golden yellow on top; centrally convex; smooth surface; glossy appearance; moist texture; easily lifted; Gram-positive (blue-violet); cocci
Sharing mode	Public welfare sharing