Bifidobacterium longum subsp. suis|134296 |BNCC

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Bifidobacterium longum subsp. sius

Culture medium	BNCC352254
Description	BBL agar medium
Composition	Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃)
Growth conditions	37°C, 24-48h, anaerobic
Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–2 mm; circular with irregular margins; opaque; white upper surface; centrally convex; smooth surface; glossy appearance; moist texture; viscous consistency; G+ (blue-violet); bacillus; purity: pure
Sharing mode	Public welfare sharing