BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 30°C; 24-48h; aerobic; |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Use forceps to break it into pieces; ③ Pipette 0.5mL sterile water into the freeze-dried vial. After fully dissolving the bacterial powder, dispense approximately 200μL onto each of two plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular, with neat edges; opaque, grayish-white upper surface; centrally raised; smooth, glossy surface; moist texture; easily liftable; G- (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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