Bifidobacterium thermophilum|134327 |BNCC

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Bifidobacterium thermophilum

Culture medium	BNCC360833
Description	BBL Fluid Medium
Composition	Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0g, pH:7.0±0.1(25℃)
Growth conditions	37°C, 48-72h, anaerobic
Subculture procedure	① Prepare 1 test tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Bifidobacterium thermophilum, a Gram-positive rod, exhibits abundant growth at the bottom of BBL liquid culture medium. Agar plate cultures show sparse growth; liquid culture is recommended.
Sharing mode	Public welfare sharing