Bacillus cereus|134491 |BNCC

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Bacillus cereus

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to break it; shatter with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2mL of bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under specified conditions; strain is ready upon growth.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter 2–4mm, circular with irregular margins; opaque, pale yellow, flat morphology; rough surface, dry texture; G+ (blue-violet), bacillus
Sharing mode	Public welfare sharing