BeNa Culture Collection
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| Culture medium | BNCC352232 |
| Description | Tryptic Soy Broth |
| Composition | Tryptone:17.0g, Soy Peptone:3.0g, NaCl:5.0g, K2HPO4:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃) |
| Growth conditions | 30°C; 18-24h; aerobic; |
| Subculture procedure | ① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2mL of bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under specified conditions; strain once the culture grows. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Golden yellow Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted |
| Sharing mode | Public welfare sharing |
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