Bacillus pontiacensis|392073 |BNCC

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Sulfitobacter pontiacus

Culture medium	BNCC353703
Description	2216E agar
Composition	Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC₆H₅O₇):0.1g, KBr:0.08g, NaCl:19.45g, MgCl₂:5.98g, Na₂SO₄:3.24g, CaCl₂:1.8g, KCl:0.55g, Na₂CO₃:0.16g, Na₂NO₃:0.0016g, Na₂HPO₄:0.008g, SrCl₂:0.034g, H₃BO₃:0.022g, Na₂SiO₃:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃)
Growth conditions	28°C; 48-72h; aerobic
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Immediately break it into pieces using forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial. After thoroughly dissolving the bacterial powder, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under the specified conditions. The culture is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with neat margins; opaque, yellow on top; centrally convex; smooth surface; moist texture; easily liftable; G- (red); bacillus
Sharing mode	Public welfare sharing