Shigella flexneri|138608 |BNCC

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Shigella flexneri

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C; 24-48h; aerobic
Subculture procedure	① Prepare 1 test tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 1-2 mm, circular, with neat edges; opaque, grayish-white on top, centrally convex, smooth surface, glossy appearance, moist texture, easily liftable; G- (red), short rod-shaped; Purity: Pure
Sharing mode	Public welfare sharing