BeNa Culture Collection
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| Culture medium | BNCC353703 |
| Description | 2216E agar |
| Composition | Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC6H5O7):0.1g, KBr:0.08g, NaCl:19.45g, MgCl2:5.98g, Na2SO4:3.24g, CaCl2:1.8g, KCl:0.55g, Na2CO3:0.16g, Na2NO3:0.0016g, Na2HPO4:0.008g, SrCl2:0.034g, H3BO3:0.022g, Na2SiO3:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃) |
| Growth conditions | 28°C; 48-72h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Break it into pieces using forceps; ③ Pipette 0.5mL sterile water into the lyophilized tube. After fully dissolving the bacterial powder, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under the specified conditions. The culture is ready for use once the strain grows. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular shape with neat margins; opaque; yellow surface; central elevation; smooth, glossy surface; moist texture; easily liftable; Gram-negative (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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